In vitro Shoot Regeneration Improvement with Thidiazuron and Benzyladenine in Rapeseed (Brassica napus L.)

An in vitro method was developed for high frequency shoot regeneration and continuous production of Brassica napus L. in large number. The different concentrations of benzyladenine (1.5, 3.0 and 4.5 mgl and thidiazuron (0, 0.15 and 0.30 mgl) were evaluated for shoot and root regeneration using hypocotyl explants with 7, 14 and 21 days old. Shoot regeneration (SR) was affected by both benzyladenine (BA) and thidiazuron (TDZ). Maximum shoots were regenerated with 4.5 mgl BA and 0.30 mgl TDZ. Shoot regeneration was highly affected by age of explants, however, 14-day-old explants were more profitable for shoot development than others. In BA concentrations, the shoot regeneration declined with the increase of age of explants. Significant differences were found in interactions of BA and TDZ concentrations and ages of explants. The maximum root regenerating plants were obtained from using of 3 mgl BA, 0.3 mgl TDZ and 21 days old explants. Rooting of regenerating shoot occurred readily on indolebutyric acid supplemented media. Rooted plantlets were successfully established in soil and developed normal fertile flowers and viable seeds.